Thermolysine adsorption on membranes at parallel flow of retentate

Enzyme surface concentration and permeate stream obtained in the membrane module with a dynamically formed biocatalyst layer were determined experimentally using two different, polymeric membranes. Correlations were formulated to calculate these values. It was found that their variability range was so big that when controlling the transmembrane pressure and turbulence of a retentate stream, the mass of used biocatalyst in membrane bioreactor could be distributed in a controlled way between both reaction zones (with the native and adsorbed enzyme) in bioreactor.